nuclear fast red  (StatLab Medical Products Inc)

Journal: Acta Physiologica (Oxford, England)

Article Title: Kisspeptin Restores Placental mTOR Signaling and Improves Glucose Homeostasis Mediators Disrupted by Maternal Hypothyroidism in Rats

doi: 10.1111/apha.70188

Figure Lengend Snippet: Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

Article Snippet: The next day, sections were kept at room temperature (2 h), and incubated with alkaline phosphatase–conjugated secondary antibody (ab6722, 1:500, Abcam, 1 h) and developed with NBT/BCIP (34 042, Thermo Scientific, 10 min) followed by Fast Red counterstaining (H‐3403, Vector Laboratories, 4 min).

Techniques: Control, Staining, Double Staining, Membrane, Diffusion-based Assay